Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Karen Cristina Fialho dos Santos; Lili Costa Maia Alencar Simões  Freitas; Antônio da Silva  Souza; Carlos Alberto da Silva Ledo

doi:10.53660/CLM-538-438

Autores

Karen Cristina Fialho dos Santos Embrapa Mandioca e Fruticultura
Lili Costa Maia Alencar Simões Freitas Universidade Federal do Recôncavo da Bahia
Antônio da Silva Souza Embrapa Mandioca e Fruticultura
Carlos Alberto da Silva Ledo Embrapa Mandioca e Fruticultura

DOI:

https://doi.org/10.53660/CLM-538-438

Palavras-chave:

Active germplasm bank, Slow growth, Tissue culture

Resumo

The objective of the present work was to evaluate in vitro growth of cassava accessions under different temperatures to improve protocols for germplasm conservation. Acclimatized chambers were tested at 18 °C, 20 °C and 22 °C, and again at 22 °C in conservation room (control) in five accessions: Mandioca João Grande (BGM1325), Fécula Branca (BGM1632), BRS Kiriris (BGM1728), S/N (121) (BGM2004) and BRS Prata (BGM2019). After 90 days of distance, the following were evaluated: shoot height (cm), number of sprouts and microcuttings, number of green and senescent leaves, fresh and dry weight of shoots and roots (g). The experiment was performed under a completely randomized design in factorial scheme 5(accessions) x 4(temperatures), with 20 replicates. All data were submitted to the F test ANOVA and evaluated by the Tukey and Scott-Knott tests at 5% probability. The control temperature promoted lower mean values for plant shoot height, with emphasis for accessions BGM1325, BGM1632 and BGM2019. In a general manner accessions BGM1325, BGM1632 and BGM2019 showed lower SH, with the first and BGM2004 outstanding with the best results for lower NSL and higher NGL.

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