Collagen extraction and physicochemical characterization of animal by-products
Extração de colágeno e caracterização físico-química de subprodutos de origem animal
Palavras-chave:
Collagen, Protein, Swim bladderResumo
Collagen is the most abundant structural protein in animals, being the main component of the skin, and can be researched and utilized by the cosmetics, pharmaceutical, food, and packaging industries. The present study investigates the extraction and quantification of collagen from three different animal sources: beef mocotó, chicken feet, and tambaqui swim bladder. Using the method of extraction in soluble acid solution (acetic acid) and precipitation in sodium chloride saline solution, collagen yields and their physicochemical properties were determined. The results revealed that the tambaqui swim bladder, especially the outer layer, presented the highest yield (39.97%), followed by chicken feet (3.49%) and bovine mocotó (1.79%). This study highlights the viability of these sources for the production of high-quality collagen, with potential applications in the cosmetics, pharmaceutical, and biomaterials industries, promoting a sustainable and economical approach to the use of animal by-products.
Downloads
Referências
ALMEIDA, J. J. Extração e caracterização de colágeno provindo de Gallus gallus domesticus: desenvolvimento de tecnologias para o tratamento de lesões cutâneas. 2018. 135 f. Dissertação (Mestrado) - Curso de Mestrado Acadêmico em Socio biodiversidade e Tecnologias Sustentáveis - MASTS Instituto de Engenharia e Desenvolvimento Sustentável – IEDS Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, 2018.
BENJAKUL, S.; KITTIPHATTANABAWON, P. Gelatin. Module in Food Science, 2018.
BRAVO, F. I.; CALVO, E.; LÓPEZ-VILLALBA, R. A.; TORRES-FUENTES, C.; MUGUERZA, B.; GARCÍA-RUIZ, A.; MORALES, D. Valorization of Chicken Slaughterhouse Byproducts to Obtain Antihypertensive Peptides. Nutrients, v. 15, p. 457, 2023.
CHEN, Y.; JIN, H.; YANG, F.; JIN, S.; LIU, C.; ZHANG, L.; HUANG, J.; WANG, S.; YAN, Z.; CAI, X.; ZHAO, R.; YU, F.; YANG, Z.; DING, G.; TANG, Y. Physicochemical antioxidant properties of giant croaker (Nibea japonica) swim bladders collagen and wound healing evaluation. International Journal of Biological Macromolecules, v. 138, p. 483-491, 2019.
COSTA, F. T.; OLIVEIRA, T. P.; DROVAL, A. A.; MARQUES, L. L. M.; FUCHS, R. H. B.; CARDOSO, F. A. R. Evaluation of physicochemical properties of Nile tilapia skin collagen extracted in acid medium. Brazilian Journal of Biology, v. 84, p. e255440, 2022.
DONG, Y.; DAI, Z. Physicochemical, Structural and Antioxidant Properties of Collagens from the Swim Bladder of Four Fish Species. Marine Drugs, v. 20, 2022.
FAUZI, M.; LOKANATHAN, Y.; AMINUDDIN, B.; RUSZYMAH, B.; CHOWDHURY, S. Ovine tendon collagen: Extraction, characterisation and fabrication of thin films for tissue engineering applications. Materials Science & Engineering C, Materials for Biological Applications, v. 68, p. 163-171, 2016.
FERREIRA, M.; GOMES, A.; GOZZO, A. Extração e caracterização de gelatina a partir de subprodutos de Tilápia do Nilo (Sarotherodon niloticus). Blucher Chemical Engineering Proceedings, v. 1, n. 3, p. 1786-1792, 2015.
GARDIM, R. B.; ALMEIDA, P. F.; GAMARRA, F. M. C.; BORINI, G. B.; SANTANA, J. C. C. Otimização da extração do colágeno de pés de frango pela metodologia da superfície de resposta. São Paulo: Blucher, 2018. ISSN 2359-1757.
GUIDA, L. M. Extração de colágeno obtido a partir de subprodutos do pirarucu (Arapaima gigas). 2019.
JU, H.; LIU, X.; ZHANG, G.; LIU, D.; YANG, Y. Comparação das características estruturais de fibrilas de colágeno nativas derivadas de tendões bovinos usando dois métodos diferentes: extração solubilizada em ácido modificado e extração auxiliada por pepsina. Materials, v. 13, 2020.
HASHEMI, S. S.; MOHAMMADI, A. A.; KABIRI, H.; HASHEMPOOR, M. R.; MAHMOODI, M.; AMINI, M.; MEHRABANI, D. The healing effect of Wharton's jelly stem cells seeded on biological scaffold in chronic skin ulcers: A randomized clinical trial. Journal of Cosmetic Dermatology, v. 18, n. 6, p. 1961-1967, 2019.
KAEWDANG, O.; BENJAKUL, S.; KAEWMANEE, T.; KISHIMURA, H. Characteristics of collagens from the swim bladders of yellowfin tuna (Thunnus albacares). Food Chemistry, v. 155, p. 264-270, 2014.
KRISHNAMOORTHI, J.; RAMASAMY, P.; SHANMUGAM, V.; SHANMUGAM, A. Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast. Biochemistry and Biophysics Reports, v. 10, p. 39-45, 2017.
KURNIASIH, R.; SWASTAWATI, F.; RIYADI, P.; RIANINGSIH, L. The influence of extraction period on the characteristics of acid soluble collagen from sea catfish (Arius thalassinus) swim bladder. IOP Conference Series: Earth and Environmental Science, v. 890, 2021..
LIN, Y.; LIU, D. Comparison of physical-chemical properties of type I collagen from different species. Food Chemistry, v. 99, p. 244-251, 2006.
LIN, K.; ZHANG, D.; MACEDO, M.; CUI, W.; SARMENTO, B.; SHEN, G. Advanced Collagen-Based Biomaterials for Regenerative Biomedicine. Advanced Functional Materials, v. 29, 2018.
MATINONG, A.; CHISTI, Y.; PICKERING, K.; HAVERKAMP, R. Collagen Extraction from Animal Skin. Biology, v. 11, 2022.
MUYONGA, J. H.; COLE, C. G. B.; DUODU, K. G. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin. Food Hydrocolloids, v. 18, n. 4, p. 581-592, 2004.
NOORZAI, S.; VERBEEK, C. J. R.; LAY, M. C. Extração de colágeno de várias fontes de resíduos de couro bovino. Waste Biomass Valor, v. 11, p. 5687–5698, 2020.
PROCÓPIO, F. R. Pesquisadores obtêm gelatina da pele do tambaqui. Embrapa, 2024..
RAHIMI, K.; ALIZADEH, M.; ABDEKHODAIE, M. Comparative study of physical properties of enzymatic in situ hydrogels based on bovine Achilles tendon collagen and rat tail collagen. In: 2020 27th National and 5th International Iranian Conference on Biomedical Engineering (ICBME). 2020. p. 143-147.
SINTHUSAMRAN, S.; BENJAKUL, S.; KISHIMURA, H. Comparative study on molecular characteristics of acid soluble collagens from skin and swim bladder of seabass (Lates calcarifer). Food Chemistry, v. 138, n. 4, p. 2435-2441, 2013.
SUN, L.; HOU, H.; LI, B.; ZHANG, Y. Characterization of acid-and pepsin-soluble collagen extracted from the skin of Nile tilapia (Oreochromis niloticus). International Journal of Biological Macromolecules, v. 99, p. 8-14, 2017.
SCHRIEBER, R.; GAREIS, H. Gelatine Handbook: Theory and Industrial Practice. Wiley-VCH, 2007.
SILVA, T. H.; MOREIRA-SILVA, J.; MARQUES, A. L.; DOMINGUES, A.; BAYON, Y.; REIS, R. L. Marine origin collagens and its potential applications. Marine Drugs, v. 12, n. 12, p. 5881-5901, 2014.
ZHANG, F.; WANG, A.; LI, Z.; HE, S.; SHAO, L. Extraction and characterization of collagen from fresh and aged grass carp skins: Comparative study on acid-soluble and pepsin-soluble collagen. Journal of Aquatic Food Product Technology, v. 29, n. 5, p. 497-508, 2020 (1).
ZHANG, T.; SUN, R.; DING, M.; LI, L.; TAO, N.; WANG, X.; ZHONG, J. Commercial cold-water fish skin gelatin and bovine bone gelatina: structural, functional, and emulsion stability differences. LWT-Food Science and Technology, v. 125, 2020 (2).
WHITE, R.; RAZGOUR, O. Emerging zoonotic diseases originating in mammals: a systematic review of effects of anthropogenic land-use change. Mammal Review, v. 50, p. 336-352, 2020.
WOO, H.; JEONG, G.; CHOI, H.; LEE, C. Characterization of Low-Molecular-Weight Collagen from Korean Native Chicken Feet Hydrolyzed Using Alcalase. Journal of Microbiology and Biotechnology, v. 33, p. 656-661, 2023.
ZIENKIEWICZ, A.; REJÓN, J. D.; DIOS ALCHÉ, J;, RODRÍGUEZ-GARCÍA, M. I.; CASTRO, A. J. A protocol for protein extraction from lipid-rich plant tissues suitable for electrophoresis. Methods Mol Biol. 2014;1072:85-91.
ZHOU, C.; YANHUA, L.; YU, X.; YANG, H.; MA, H.; YAGOUB, A.; CHENG, Y.; HU, J.; OTU, P. Extraction and characterization of chicken feet soluble collagen. LWT-Food Science and Technology, v. 74, p. 145-153, 2016.