Semen microorganisms’ prevalence in tabapuã bulls
Prevalência de microrganismos no sêmen de touros tabapuã
Palavras-chave:
Microbiology, Ejaculate, Bovines, AntimicrobialsResumo
The study objective was to characterize the microbiological aspects of raw semen from Tabapuã bulls kept in a semen production center. Five Tabapuã bulls were used, and four ejaculates were collected using the artificial vagina method. Bacterial colonies were quantified and identified by morphotintorial analysis (Gram staining) and identification tests for bacterial isolates. Among the twenty semen samples collected, bacteria genera were identified: Citrobacter sp. (3.03%); Corynebacterium sp. (3.03%); Enterobacter sp. (9.09%); Escherichia sp. (9.09%); Klebsiella sp. (9.09%); Providencia sp. (3.03%); Pseudomonas sp. (12.12%); Staphylococcus sp. (45.46%) and Streptococcus sp. (6.06%). It was possible to observe and identify the microbial agents present in the semen samples.
Downloads
Referências
AL-KASS, Z. et al. Bacteria Detected in the Genital Tract, Semen or Pre-Ejaculatory Fluid of Swedish Stallions from 2007 to 2017. Acta Veterinaria Scandinavica, v.61, n.1, p.4-9, 2019.
BRASIL. Agência Nacional de Vigilância Sanitária. Detecção e Identificação de Bactérias de Importância Médica Módulo V. Brasília, DF, 2013. 93p.
BRASIL. Agência Nacional de Vigilância Sanitária. Clinical and Laboratory Standards Institute. Normas de Desempenho Para Testes de Sensibilidade Antimicrobiana. 15º Suplemento Informativo. Brasília, DF, v.25, 2005, 177p.
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento - BINAGRI SISLEGIS. Instrução Normativa 48/2003. Brasília, DF, 2003, 4p.
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento - Instrução Normativa 10/2017. Brasília, DF, 2001, 4p.
DINIZ, J.H.W. et al. The Importance of Updated Criteria in Breeding Soundness Examination of Commercial Nellore Bulls for the Herd Reproductive Improvement. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 73, n. 2, p. 285-292, 2021.
GOULARTE, K.L. et al. Antibiotic Resistance in Microorganisms Isolated in a Bull Semen Stud. Reproduction in Domestic Animals, v. 55, n. 3, p. 318-324, 2020.
INDEX ASBIA / Associação Brasileira de Inseminação Artificial, 1º Semestre / 2021, Universidade de São Paulo: ASBIA, 2021. Disponível em: . Acesso em 29 ago. 2021.
KILBURN, C. et al. Antimicrobial Resistance in Some Gram-Negative Bacteria Isolated from the Bovine Ejaculate. Reproduction in Domestic Animals, v.48, n.3, p.525-528, 2013.
Manual para exame andrológico e avaliação de sêmen animal / Colégio Brasileiro de Reprodução Animal, 3ª ed., Belo Horizonte: CBRA 2013. Disponível em: Acesso em 01 out. 2021.
MITRA, J. et al. Microbiological Evaluation of Bovine Frozen Semen Samples in West Bengal , India. Exploratory Animal and Medical Research, v.6, n.2, p.185-191, 2016.
MARTINS, C.R.F. et al. Ministério da Saúde. Técnica de Coloração de GRAM. Brasília, DF, 2001, 67p.
OIE. Word organization for animal health. Terrestrial Animal Health Code. 13ª ed., Paris: OIE 2010. Disponível em: <https://www.oie.int/>. Acesso em 29 dez. 2021
REDA, A.A. et al. Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia. Veterinary Medicine International, p.1-11, 2020.
ZHANG, J. et al. Genomic Sequencing Reveals the Diversity of Seminal Bacteria and Relationships to Reproductive Potential in Boar Sperm. Frontiers in Microbiology, v.11, p.1-16, 2020.
