Morphometric analysis of rat femurs submitted to the use of dexamethasone and the consumption of curcumin and symbiotics
Análise morfométrica de fêmures de ratos submetidos ao uso de dexametasona e ao consumo de curcumina e simbiótico
DOI:
https://doi.org/10.53660/CLM-3627-24M29CPalavras-chave:
Spongy Bone, Symbiotic, Femoral Head, Curcumin, GlucocorticoidResumo
The aim was to analyze whether the deleterious effects of dexamethasone on bone can be prevented or mitigated by using curcumin and the symbiotic concomitantly. The rats were divided into control (CT), dexamethasone (D), dexamethasone curcumin (DC), dexamethasone symbiotic (DS) and dexamethasone symbiotic/curcumin (DCS) groups. The thickness, area, and area fraction of the bone trabeculae of the femoral head were measured. The CT group showed greater trabecular thickness when compared to the D and DS groups. The thicknesses of the DC and DCS groups were greater than those of the DS group. The area and area fraction were smaller in groups D, DC and DS compared to CT. It can be concluded that curcumin, when consumed in isolation, had a slight effect on maintaining the thickness of trabecular bone. Considering its action on the structuring of the trabecular area, it is not possible to establish a clear relationship between its benefit for bone preservation and its interference with bone cell activity.
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Referências
ADAMI, G.; SAAG, K. G. Glucocorticoid-induced osteoporosis: 2019 concise clinical review. Osteoporosis International, v. 30, n. 6, p. 1145–1156, 1 jun. 2019.
BÖCKER, W. et al. Short-term glucocorticoid treatment causes spinal osteoporosis in ovariectomized rats. European Spine Journal, v. 23, n. 11, p. 2437–2448, nov. 2014.
CASTANIA, V. A. et al. Advantages of a combined method of decalcification compared to EDTA. Microscopy Research and Technique, v. 78, n. 2, p. 111–118, fev. 2015.
CHEN, S. M. et al. Dexamethasone Down-regulates Osteocalcin in Bone Cells through Leptin Pathway. International Journal of Medical Sciences, v. 15, n. 5, p. 507–516, 2018.
CHEN, W. et al. Dexamethasone inhibits the differentiation of rat tendon stem cells into tenocytes by targeting the scleraxis gene. The Journal of Steroid Biochemistry and Molecular Biology, v. 152, p. 16–24, ago. 2015.
CHEN, Z. et al. Curcumin alleviates glucocorticoid-induced osteoporosis by protecting osteoblasts from apoptosis in vivo and in vitro. Clinical and Experimental Pharmacology and Physiology, v. 43, n. 2, p. 268–276, 2016a.
CHEN, Z. et al. Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway. International Journal of Molecular Medicine, v. 37, n. 2, p. 329–338, 1 fev. 2016b.
CIRANO, F. R. et al. Effect of curcumin on bone tissue in the diabetic rat: repair of peri-implant and critical-sized defects. International Journal of Oral and Maxillofacial Surgery, v. 47, n. 11, p. 1495–1503, nov. 2018.
DE OLIVEIRA, J. A. G. T. et al. Analysis of the effects of curcumin and symbiotic consumption on bones of rats submitted to the use of dexamethasone. Life Sciences, v. 304, p. 120690, 1 set. 2022.
ILESANMI-OYELERE, B. L.; KRUGER, M. C. The Role of Milk Components, Pro-, Pre-, and Synbiotic Foods in Calcium Absorption and Bone Health Maintenance. Frontiers in Nutrition, v. 7, p. 578702, 2020.
MARKOWIAK, P.; ŚLIŻEWSKA, K. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients, v. 9, n. 9, p. E1021, 15 set. 2017.
SCHOLZ-AHRENS, K. E. et al. Effects of probiotics, prebiotics, and synbiotics on mineral metabolism in ovariectomized rats — impact of bacterial mass, intestinal absorptive area and reduction of bone turn-over. NFS Journal, v. 3, p. 41–50, 1 ago. 2016.
TAGLIARI, E. et al. The impact of the use of symbiotics in the progression of nonalcoholic fatty liver disease in a rat model. Arquivos brasileiros de cirurgia digestiva, v. 30, n. 3, p. 211–215, set. 2017.
WEINSTEIN, R. S. Glucocorticoid-induced osteonecrosis. Endocrine, v. 41, n. 2, p. 183–190, abr. 2012.
YANG, M.-W. et al. Curcumin improves bone microarchitecture and enhances mineral density in APP/PS1 transgenic mice. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, v. 18, n. 2–3, p. 205–213, 15 jan. 2011.
ZHOU, R.-X. et al. Linking the relation between gut microbiota and glucocorticoid-induced osteoporosis. Journal of Bone and Mineral Metabolism, v. 41, n. 2, p. 145–162, mar. 2023.
