Effects of curcumin on the calcaneal tendon of rats under concomitant use of dexamethasone
Efeitos da curcumina no tendão calcâneo de ratos sob uso concomitante de dexametasona
Palavras-chave:
Tendon, Dexamethasone, Curcumin, Strength, DeformationResumo
The aim of this study was to evaluate whether the use of dexamethasone would be harmful to the calcaneal tendon of healthy Wistar rats and to verify whether the concomitant consumption of curcumin could mitigate this possible impairment. The animals were divided into healthy control group (CT - without dexamethasone and curcumin), control dexamethasone group (DEXA - with dexamethasone and without curcumin) and dexamethasone curcumin group (DEXA-CURC - with dexamethasone and curcumin). The administration of dexamethasone occurred for 5 weeks (3 times a week) and simultaneously the animals consumed curcumin during that period. The structural and material properties of tendons were analyzed. Absolute and relative deformations demonstrated lower values in the DEXA group in relation to the other groups, which were similar to each other. Although DEXA tendons resist the same maximum forces and tension, they have a lower capacity to deform. In contrast, the data showed that curcumin mitigated the stiffness since the DEXA-CURC group deformed in a similar way to the CT group. It is concluded that the consumption of curcumin favored the attenuation of the side effect of the use of dexamethasone.
Downloads
Referências
BLOMGRAN, P.; HAMMERMAN, M.; ASPENBERG, P. Systemic corticosteroids improve tendon healing when given after the early inflammatory phase. Scientific Reports, v. 7, n. 1, p. 12468, 29 set. 2017.
BOBZIN, L. et al. Development and maintenance of tendons and ligaments. Development (Cambridge, England), v. 148, n. 8, 15 abr. 2021.
BUCHANAN, C. I.; MARSH, R. L. Effects of long-term exercise on the biomechanical properties of the Achilles tendon of guinea fowl. Journal of Applied Physiology (Bethesda, Md.: 1985), v. 90, n. 1, p. 164–171, jan. 2001.
CHEN, W. et al. Dexamethasone inhibits the differentiation of rat tendon stem cells into tenocytes by targeting the scleraxis gene. The Journal of Steroid Biochemistry and Molecular Biology, v. 152, p. 16–24, ago. 2015.
CIRANO, F. R. et al. Effect of curcumin on bone tissue in the diabetic rat: repair of peri-implant and critical-sized defects. International Journal of Oral and Maxillofacial Surgery, v. 47, n. 11, p. 1495–1503, nov. 2018.
COMPSTON, J. Glucocorticoid-induced osteoporosis: an update. Endocrine, v. 61, n. 1, p. 7–16, jul. 2018.
CONNIZZO, B. K.; YANNASCOLI, S. M.; SOSLOWSKY, L. J. Structure-function relationships of postnatal tendon development: a parallel to healing. Matrix Biology: Journal of the International Society for Matrix Biology, v. 32, n. 2, p. 106–116, 11 mar. 2013.
DE CÁSSIA MARQUETI, R. et al. Resistance training minimizes the biomechanical effects of aging in three different rat tendons. Journal of Biomechanics, v. 53, p. 29–35, 28 fev. 2017.
EEKHOFF, J. D.; FANG, F.; LAKE, S. P. Multiscale mechanical effects of native collagen cross-linking in tendon. Connective Tissue Research, v. 59, n. 5, p. 410–422, set. 2018.
GE, Z. et al. Downregulation of type I collagen expression in the Achilles tendon by dexamethasone: a controlled laboratory study. Journal of Orthopaedic Surgery and Research, v. 15, n. 1, p. 70, 24 fev. 2020.
GRACEY, E. et al. Tendon and ligament mechanical loading in the pathogenesis of inflammatory arthritis. Nature Reviews. Rheumatology, v. 16, n. 4, p. 193–207, abr. 2020.
GÜLEÇ, A. et al. Effect of curcumin on tendon healing: an experimental study in a rat model of Achilles tendon injury. International Orthopaedics, v. 42, n. 8, p. 1905–1910, ago. 2018.
GUZZONI, V.; SELISTRE-DE-ARAÚJO, H. S.; MARQUETI, R. DE C. Tendon remodeling in response to resistance training, anabolic androgenic steroids and aging. Cells, v. 7, n. 12, 7 dez. 2018.
JIANG, D. et al. Curcumin improves tendon healing in rats: a histological, biochemical, and functional evaluation. Connective Tissue Research, v. 57, n. 1, p. 20–27, 2016.
KORNTNER, S. et al. A high-glucose diet affects Achilles tendon healing in rats. Scientific Reports, v. 7, n. 1, p. 780, 10 abr. 2017.
LUCINDA, L. M. F. et al. The effect of the Ginkgo biloba extract in the expression of Bax, Bcl-2 and bone mineral content of Wistar rats with glucocorticoid-induced osteoporosis. Phytotherapy research: PTR, v. 27, n. 4, p. 515–520, abr. 2013.
NAKAGAKI, W. R. et al. Biomechanical and biochemical properties of chicken calcaneal tendon under effect of age and nonforced active exercise. Connective Tissue Research, v. 48, n. 5, p. 219–228, 2007.
POULSEN, R. C. et al. Glucocorticoids induce senescence in primary human tenocytes by inhibition of sirtuin 1 and activation of the p53/p21 pathway: in vivo and in vitro evidence. Annals of the Rheumatic Diseases, v. 73, n. 7, p. 1405–1413, jul. 2014.
NAKAGAKI, W. R. et al. The effect of age and spontaneous exercise on the biomechanical and biochemical properties of chicken superficial digital flexor tendon. Connective Tissue Research, v. 51, n. 4, p. 265–273, ago. 2010.
SHADWICK, R. E. Elastic energy storage in tendons: mechanical differences related to function and age. Journal of Applied Physiology (Bethesda, Md.: 1985), v. 68, n. 3, p. 1033–1040, mar. 1990.
TAGUCHI, T. et al. Quantitative and qualitative change of collagen of achilles tendons in rats with systemic administration of glucocorticoids. Foot & Ankle International, v. 37, n. 3, p. 327–333, mar. 2016.
WANG, J. H.-C.; GUO, Q.; LI, B. Tendon biomechanics and mechanobiology--a minireview of basic concepts and recent advancements. Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists, v. 25, n. 2, p. 133–140, jun. 2012.
ZHANG, J.; KEENAN, C.; WANG, J. H.-C. The effects of dexamethasone on human patellar tendon stem cells: implications for dexamethasone treatment of tendon injury. Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society, v. 31, n. 1, p. 105–110, jan. 2013.
ZHANG, W. et al. Controlled release of curcumin from curcumin-loaded nanomicelles to prevent peritendinous adhesion during Achilles tendon healing in rats. International Journal of Nanomedicine, v. 11, p. 2873–2881, 2016.
