Evaluation of in vitro Schistosomicidal and Trypanocidal Activity of Leaf Extracts of Hancornia speciosa

Avaliação da Atividade Esquistossomicida e Tripanocida in vitro dos Extratos Foliares de Hancornia speciosa

Autores

Palavras-chave:

Brazilian Cerrado, Mangabeira, T. cruzi, S. mansoni, Phytochemical analysis

Resumo

Medicinal plants are viable for threating various diseases, as the metabolites they contain demonstrate a great diversity of biological activities. In this study, we conducted a phytochemical analysis and evaluated of the in vitro antiparasitic activity of extracts from the leaves of Hancornia speciosa, popularly known as mangabeira, against Trypanossoma cruzi and Schistosoma mansoni parasites. We collected the leaves of this species in the municipality of Urutaí - GO, and obtained an ethanolic extract, in which classes of organic acid compounds, coumarins, reducing sugars, catechin tannins, foaming saponins and flavonoids were identified by phytochemical analysis. The antiparasitic evaluation of the fractions obtained showed that H. speciosa was ineffective against S. mansoni. However, the hexane fraction of the extract of mangaba is moderately effective against T. cruzi, demonstrating IC50 values of 31.71 mg mL-1. This is the first time the antiparasitic activity of H. speciosa has been described in the literature.

Downloads

Não há dados estatísticos.

Biografia do Autor

Herbert Júnior Dias, Instituto Federal Goiano - Campus Urutaí

Possui bacharelado em Química pela Universidade de Franca (2010), e Mestrado em Ciências pela mesma universidade (2013), onde desenvolveu projeto na área de produtos naturais, com ênfase na aplicação de espectrometria de massas para a determinação de padrões de fragmentação para fins de elucidação estrutural; e análise de óleos essenciais empregando técnicas hifenadas e suas respectivas atividades biológicas. Concluiu a sua formação de docente (Licenciatura) na mesma universidade (2015). Concluiu seu doutorado no Programa de Pós-graduação em Química na FFCLRP-USP (2018), onde desenvolveu projeto que envolveu síntese de derivados de compostos benzofurânicos e estudo de fragmentação empregando espectrometria de massas sequencial. Atualmente é professor no Instituto Federal de Educação, Ciência e Tecnologia Goiano - Campus Urutaí, membro do Núcleo de Química e do Programa de Pós-Graduação em Recursos Naturais do Cerrado (PPG-CRENAC). Desenvolve projetos na área de Química Orgânica, Química Analítica, Química dos Produtos Naturais, e Educação em Ciências.

Referências

ASSUMPÇÃO, C. F.; BACHIEGA, P.; MORZELLE, M. C.; NELSON, D. L.; NDIAYE, E. A.; RIOS, A. DE O.; SOUZA, É. C. DE. Characterization, antioxidant potential and cytotoxic study of mangaba fruits. Ciência Rural, v. 44, n. 7, p. 1297–1303, jul. 2014.

BASTOS, K.; DIAS, C.; NASCIMENTO, Y.; SILVA, M. DA; LANGASSNER, S.; WESSJOHANN, L.; TAVARES, J. Identification of phenolic compounds from Hancornia speciosa (Apocynaceae) leaves by UHPLC Orbitrap-HRMS. Molecules, v. 22, n. 1, p. 143, 15 jan. 2017.

BITENCOURT, M. A. O.; TORRES-RÊGO, M.; SOUZA LIMA, M. C. J. DE; FURTADO, A. A.; AZEVEDO, E. P. DE; EGITO, E. S. T. DO; SILVA-JÚNIOR, A. A. DA; ZUCOLOTTO, S. M.; FERNANDES-PEDROSA, M. DE F. Protective effect of aqueous extract, fractions and phenolic compounds of Hancornia speciosa fruits on the inflammatory damage in the lungs of mice induced by Tityus serrulatus envenomation. Toxicon, v. 164, p. 1–9, jun. 2019.

BORGES, A. R.; AIRES, J. R. DE A.; HIGINO, T. M. M.; MEDEIROS, M. DAS G. F. DE; CITÓ, A. M. DAS G. L.; LOPES, J. A. D.; FIGUEIREDO, R. C. B. Q. DE. Trypanocidal and cytotoxic activities of essential oils from medicinal plants of Northeast of Brazil. Experimental Parasitology, v. 132, n. 2, p. 123–128, out. 2012.

BORLAUG, N. E. Feeding a world of 10 billion people: The miracle ahead. Biotechnology & Biotechnological Equipment, v. 11, n. 3–4, p. 3–13, 15 jan. 1997.

BÖTTGER, A.; VOTHKNECHT, U.; BOLLE, C.; WOLF, A. Plant secondary metabolites and their general function in plants. Em: Lessons on Caffeine, Cannabis & Co . v. 1, p. 3–17.

BRASIL. Deforestation in the Cerrado in 2018. Disponível em: <http://combateaodesmatamento.mma.gov.br/images/Doc_ComissaoExecutiva/Balanco-PPCDAm-e-PPCerrado_2018.pdf>. Acesso em: 31 out. 2022.

BRUM, T. F. DE; ZADRA, M.; FROEDER, A. L. F.; BOLIGON, A. A.; FROHLICH, J. K.; ATHAYDE, M. L. Phytochemical analysis of the Vitex megapotamica (Sprengel) Moldenke leaves. Saúde (Santa Maria), v. 37, n. 2, p. 101, 26 abr. 2012.

BRUNING, M. C. R.; MOSEGUI, G. B. G.; VIANNA, C. M. DE M. The use of phytotherapy and medicinal plants in primary healthcare units in the cities of Cascavel and Foz do Iguaçu - Paraná: the viewpoint of health professionals. Ciência & Saúde Coletiva, v. 17, n. 10, p. 2675–2685, out. 2012.

CARVALHO, F. A.; SILVA, B. C.; ODA, F. B.; JUNIOR, J. C. P.; CREVELIN, E. J.; CROTTI, A. E. M.; CALDAS, L. G. M.; SANTOS, A. G. In vitro schistosomicidal activities of the leaf extracts from Casearia sylvestris varieties. Chemistry & Biodiversity, v. 19, n. 2, 11 fev. 2022.

CASTRO OLIVEIRA, H. W.; VIVEIRO, A. A. Cerrado and medicinal plants: some reflections about use and conservation. Ensino, Saude e Ambiente, v. 5, n. 3, p. 102–120, 30 out. 2013.

CHAVES, S. M.; PANONTIN, J. F.; SEIBERT, C. S.; SCAPIN, E. Screening fitoquímico da folha e caule da Hancornia speciosaGomes (mangabeira) com finalidade de bioprospecção cosmética. Brazilian Journal of Health Review, v. 3, n. 1, p. 1212–1222, 2020.

COSTA, E. S.; HIRUMA-LIMA, C. A.; LIMA, E. O.; SUCUPIRA, G. C.; BERTOLIN, A. O.; LOLIS, S. F.; ANDRADE, F. D. P.; VILEGAS, W.; SOUZA-BRITO, A. R. M. Antimicrobial activity of some medicinal plants of the Cerrado, Brazil. Phytotherapy Research, v. 22, n. 5, p. 705–707, maio 2008.

CRIBB, D. M.; CLARKE, N. E.; DOI, S. A. R.; VAZ NERY, S. Differential impact of mass and targeted praziquantel delivery on schistosomiasis control in school-aged children: A systematic review and meta-analysis. PLOS Neglected Tropical Diseases, v. 13, n. 10, p. e0007808, 11 out. 2019.

DANTAS, L. A.; FARIA, P. S. A.; DÁRIO, B. M. M.; ARANTES, A. L. M.; SILVA, F. G.; AVILA, R. G.; PEREIRA, P. S.; NETO, A. R. The impact of carbon source on cell growth and the production of bioactive compounds in cell suspensions of Hancornia speciosa Gomes. Scientific Reports, v. 11, n. 1, p. 24315, 21 dez. 2021.

FERRARI, M.; FORNASIERO, M. C.; ISETTA, A. M. MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. Journal of Immunological Methods, v. 131, n. 2, p. 165–172, ago. 1990.

GARCIA, A. P.; FOX, L. M. Parasitic Diseases. Em: The MassGeneral Hospital for Children Handbook of Pediatric Global Health. New York, NY: Springer New York, 2014. p. 287–301.

GARCÍA-HUERTAS, P.; CARDONA-CASTRO, N. Advances in the treatment of Chagas disease: Promising new drugs, plants and targets. Biomedicine & Pharmacotherapy, v. 142, p. 112020, out. 2021.

JÁCOME, M. C. M. B.; PADILHA, C. E. DE A.; ARRAIS, M. R. DO N.; LEITÃO, A. L. O. DE S.; SOUSA JÚNIOR, F. C.; SANTOS, E. S. Valorization of mangaba residue (Hancornia speciosa Gomes) for polygalacturonase production from Aspergillus niger IOC 4003 and fabrication of active chitosan films. Biomass Conversion and Biorefinery, v. 12, n. 9, p. 4069–4080, 10 set. 2022.

KATSUNO, K.; BURROWS, J. N.; DUNCAN, K.; HUIJSDUIJNEN, R. H. VAN; KANEKO, T.; KITA, K.; MOWBRAY, C. E.; SCHMATZ, D.; WARNER, P.; SLINGSBY, B. T. Hit and lead criteria in drug discovery for infectious diseases of the developing world. Nature Reviews Drug Discovery, v. 14, n. 11, p. 751–758, 5 nov. 2015.

KIM, M.-J.; SON, S.-Y.; JEON, S.-G.; KIM, J.-G.; LEE, C.-H. Metabolite profiling of Dioscorea (Yam) leaves to identify bioactive compounds reveals their potential as renewable resources. Plants, v. 10, n. 8, p. 1751, 23 ago. 2021.

KLINK, C. A.; MACHADO, R. B. Conservation of the Brazilian Cerrado. Conservation Biology, v. 19, n. 3, p. 707–713, jun. 2005.

LI, Z.; KONG, D.; LIU, Y.; LI, M. Pharmacological perspectives and molecular mechanisms of coumarin derivatives against virus disease. Genes & Diseases, v. 9, n. 1, p. 80–94, jan. 2022.

LIU, Y.-P.; YAN, G.; GUO, J.-M.; LIU, Y.-Y.; LI, Y.-J.; ZHAO, Y.-Y.; QIANG, L.; FU, Y.-H. Prenylated Coumarins from the Fruits of Manilkara zapota with Potential Anti-inflammatory Effects and Anti-HIV Activities. Journal of Agricultural and Food Chemistry, v. 67, n. 43, p. 11942–11947, 30 out. 2019.

LOPES, T. A.; NOBUSHIGE, S. Y. L.; SILVA, A. P. S.; GOVEIA, C. DE O.; ENK, M. J.; SAMPAIO, I.; SALES, J. B. DE L.; RODRIGUES FILHO, L. F. DA S. Distribution and identification of the genus Biomphalaria Preston (1910): important insights into the epidemiology of Schistosomiasis in the Amazon region. Revista da Biologia, v. 17, n. 2, p. 31–37, fev. 2017.

MARIN, R. M.; GARCIA, J. C. L.; GARCIA, R. M. B.; ALARCÓN, A. B. Phytochemical screening and isolation of triterpenes and sterols from leaves of Clusia minor L. Revista Cubana de Plantas Medicinales, v. 23, n. 3, p. 1–8, 2018.

MARINHO, T. A.; OLIVEIRA, M. G.; MENEZES-FILHO, A. C. P.; CASTRO, C. F. S.; OLIVEIRA, I. M. M.; BORGES, L. L.; MELO-REIS, P. R.; SILVA-JR, N. J. Phytochemical characterization, and antioxidant and antibacterial activities of the hydroethanolic extract of Anadenanthera peregrina stem bark. Brazilian Journal of Biology, v. 82, 2022.

MARTINS, M. H. G. et al. Schistosomicidal effects of the essential oils of Citrus limonia and Citrus reticulata against Schistosoma mansoni. Chemistry and Biodiversity, v. 14, n. 1, 1 jan. 2017.

MATOS, F. J. A. Introdução à Fitoquímica Experimental. 2a Edition ed. Fortaleza: Edições UFC, 1988. v. 1

MELO, A. C. O.; BONILLA, O. H.; LUCENA, E. M. P. DE. Latex of Apocynaceae family plants: A review. Research, Society and Development, v. 10, n. 8, p. e13910817192, 8 jul. 2021.

MENEZES FILHO, A. C. P. DE; OLIVEIRA FILHO, J. G. DE; CHRISTOFOLI, M.; SOUSA, C. F. DE. Antioxidant activity, total phenolics content, carotenoids and provitamin A in vegetables extracts from Cerrado Goiano. UNICIÊNCIAS, v. 22, n. 1, p. 28, 6 set. 2018.

MORAES, T. DE M.; RODRIGUES, C. M.; KUSHIMA, H.; BAUAB, T. M.; VILLEGAS, W.; PELLIZZON, C. H.; BRITO, A. R. M. S.; HIRUMA-LIMA, C. A. Hancornia speciosa: Indications of gastroprotective, healing and anti-Helicobacter pylori actions. Journal of Ethnopharmacology, v. 120, n. 2, p. 161–168, nov. 2008.

MÜLLER KRATZ, J.; GARCIA BOURNISSEN, F.; FORSYTH, C. J.; SOSA-ESTANI, S. Clinical and pharmacological profile of benznidazole for treatment of Chagas disease. Expert Review of Clinical Pharmacology, v. 11, n. 10, p. 943–957, 3 out. 2018.

NDJONKA, D.; RAPADO, L.; SILBER, A.; LIEBAU, E.; WRENGER, C. Natural products as a source for treating neglected parasitic Diseases. International Journal of Molecular Sciences, v. 14, n. 2, p. 3395–3439, 6 fev. 2013.

NETO, G. A. L.; KAFFASHI, S.; LUIZ, W. T.; FERREIRA, W. R.; DIAS DA SILVA, Y. S. A.; PAZIN, G. V.; VIOLANTE, I. M. P. Quantification of secondary metabolites and antimicrobial and antioxidant activities of some medicinal plants from the Cerrado of the Mato Grosso. Revista Brasileira de Plantas Medicinais, v. 17, n. 4 suppl 3, p. 1069–1077, 2015.

NETO, L. S.; MORAES-SOUZA, R. Q.; SOARES, T. S.; PINHEIRO, M. S.; LEAL-SILVA, T.; HOFFMANN, J. C.; AMÉRICO, M. F.; CAMPOS, K. E.; DAMASCENO, D. C.; VOLPATO, G. T. A treatment with a boiled aqueous extract of Hancornia speciosa Gomes leaves improves the metabolic status of streptozotocin-induced diabetic rats. BMC Complementary Medicine and Therapies, v. 20, n. 1, p. 114, 17 dez. 2020.

NEWMAN, D. J.; CRAGG, G. M. Natural Products as sources of new drugs over the nearly four decades from 01/1981 to 09/2019. Journal of Natural Products, v. 83, n. 3, p. 770–803, 27 mar. 2020.

PAGOTTI, M. C.; CANDIDO, A. C. B. B.; MARÇAL, M. G.; VIEIRA, T. M.; GROPPO, M.; SILVA, M. L. A.; FERREIRA, D. S.; ESPERANDIM, V. R.; CROTTI, A. E. M.; MAGALHÃES, L. G. Trypanocidal activity of Dysphania ambrosioides , Lippia alba , and Tetradenia riparia essential oils against Trypanosoma cruzi. Chemistry & Biodiversity, v. 18, n. 12, 20 dez. 2021a.

PAGOTTI, M. C.; CANDIDO, A. C.; MARÇAL, M. G.; VIEIRA, T. M.; GROPPO, M.; SILVA, M. L.; FERREIRA, D.S.; ESPERANDIM, V.R.; CROTTI, A.E.M.; MAGALHÃES, L. G. Trypanocidal Activity of Dysphania ambrosioides , Lippia alba , and Tetradenia riparia Essential Oils against Trypanosoma cruzi. Chemistry & Biodiversity, v. 18, n. 12, 20 dez. 2021b.

PANONTIN, J. F.; BARBOSA, R. DOS S.; ISAAC, V.; SEIBERT, C. S.; SCAPIN, E. Chemical composition, antioxidant activity and development of a facial serum formulation from the extract of Hancornia speciosa. Natural Product Research, v. 36, n. 23, p. 6121–6125, 2 dez. 2022.

PANONTIN, J. F.; NERES, R. P.; FERNANDES, R. DE M. N.; SCAPIN, E.; SEIBERTCARLA SIMONE. Chemical characterization and toxicological analyses of hydroalcoholic extracts from the stem and leaves of mangabeira (Hancornia speciosa Gomes) as a guide for the development of green cosmetics. Journal of Medicinal Plants Research, v. 15, n. 8, p. 366–379, 31 ago. 2021.

PARACATU, L. C.; FARIA, C. M. Q. G.; QUINELLO, C.; RENNÓ, C.; PALMEIRA, P.; ZERAIK, M. L.; FONSECA, L. M. DA; XIMENES, V. F. Caffeic acid phenethyl ester: Consequences of its hydrophobicity in the oxidative functions and cytokine release by leukocytes. Evidence-Based Complementary and Alternative Medicine, v. 2014, p. 1–13, 2014.

PEARCE, E. J.; MACDONALD, A. S. The immunobiology of schistosomiasis. Nature Reviews Immunology, v. 2, n. 7, p. 499–511, jul. 2002.

RAMIREZ, B.; BICKLE, Q.; YOUSIF, F.; FAKOREDE, F.; MOURIES, M.-A.; NWAKA, S. Schistosomes: challenges in compound screening. Expert Opinion on Drug Discovery, v. 2, n. sup1, p. S53–S61, 5 out. 2007.

RAMOS-MORALES, E.; LA FUENTE, G. DE; DUVAL, S.; WEHRLI, C.; BOUILLON, M.; LAHMANN, M.; PRESKETT, D.; BRAGANCA, R.; NEWBOLD, C. J. Antiprotozoal effect of saponins in the rumen can be enhanced by chemical modifications in their structure. Frontiers in Microbiology, v. 08, 16 mar. 2017.

RIBEIRO NETO, J. A.; PIMENTA TARÔCO, B. R.; BATISTA DOS SANTOS, H.; THOMÉ, R. G.; WOLFRAM, E.; MACIEL DE A RIBEIRO, R. I. Using the plants of Brazilian Cerrado for wound healing: From traditional use to scientific approach. Journal of Ethnopharmacology, v. 260, p. 112547, out. 2020.

RÍOS, Y. K.; OTERO, A. C.; MUÑOZ, D. L.; ECHEVERRY, M.; ROBLEDO, S. M.; YEPES, M. A. Actividad citotóxica y leishmanicida in vitro del aceite esencial de manzanilla (Matricaria chamomilla). Revista Colombiana de Ciencias Químico - Farmacéuticas, v. 37, n. 2, p. 200–211, 2008.

RODRIGUES, C. M.; RINALDO, D.; SANTOS, L. C. DOS; MONTORO, P.; PIACENTE, S.; PIZZA, C.; HIRUMA-LIMA, C. A.; BRITO, A. R. M. S.; VILEGAS, W. Metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry for the characterization of proanthocyanidins from the barks of Hancornia speciosa. Rapid Communications in Mass Spectrometry, v. 21, n. 12, p. 1907–1914, 30 jun. 2007.

SANTOS, U. P.; CAMPOS, J. F.; TORQUATO, H. F. V.; PAREDES-GAMERO, E. J.; CAROLLO, C. A.; ESTEVINHO, L. M.; PICOLI SOUZA, K. DE; SANTOS, E. L. DOS. Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa Gomes. PLOS ONE, v. 11, n. 12, p. e0167531, 1 dez. 2016.

SANTOS, U. P.; CAMPOS, J. F.; TORQUATO, H. F. V.; PAREDES-GAMERO, E. J.; CAROLLO, C. A.; ESTEVINHO, L. M.; PICOLI SOUZA, K. DE; SANTOS, E. L. DOS. Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa Gomes. Plos One, v. 11, n. 12, p. e0167531, 1 dez. 2016.

SILVA, M. L. A. E et al. In vitro Activities of Pfaffia glomerata Root Extract, Its Hydrolyzed Fractions and Pfaffic Acid Against Trypanosoma cruzi Trypomastigotes. Chemistry & Biodiversity, v. 14, n. 1, p. e1600175, jan. 2017.

SILVA, T. F. DA; COELHO, M. R. R.; VOLLÚ, R. E.; VASCONCELOS GOULART, F. R. DE; ALVIANO, D. S.; ALVIANO, C. S.; SELDIN, L. Bacterial community associated with the trunk latex of Hancornia speciosa Gomes (Apocynaceae) grown in the northeast of Brazil. Antonie van Leeuwenhoek, v. 99, n. 3, p. 523–532, 20 mar. 2011.

SIMÕES, C. M. O.; SCHENKEL, E. P.; MELLO, J. C. P.; MENTZ, L. A.; PETROVICK, P. R. Farmacognosia: Do Produto Natural ao Medicamento. 1st. ed. Porto Alegre: Artmed, 2016. v. 1

SOUSA CARVALHO, M. S. et al. Phytochemical screening, extraction of essential oils and antioxidant activity of five species of unconventional vegetables. American Journal of Plant Sciences, v. 06, n. 16, p. 2632–2639, 2015.

SOUZA, F. G. DE; FIGUEIREDO, R. W. DE; ALVES, R. E.; MAIA, G. A.; ARAÚJO, I. A. DE. Postharvest quality of fruits from different mangabeira clones (Hancornia speciosa Gomes). Ciência e Agrotecnologia, v. 31, n. 5, p. 1449–1454, out. 2007.

SOUZA, L. I. O.; BEZZERA-SILVA, P. C.; AMARAL FERRAZ NAVARRO, D. M.; SILVA, A. G.; SANTOS CORREIA, M. T.; SILVA, M. V.; FIGUEIREDO, R. C. B. Q. DE. The chemical composition and trypanocidal activity of volatile oils from Brazilian Caatinga plants. Biomedicine & Pharmacotherapy, v. 96, p. 1055–1064, dez. 2017.

TRAINA, M. I.; HERNANDEZ, S.; SANCHEZ, D. R.; DUFANI, J.; SALIH, M.; ABUHAMIDAH, A. M.; OLMEDO, W.; BRADFIELD, J. S.; FORSYTH, C. J.; MEYMANDI, S. K. Prevalence of Chagas Disease in a U.S. population of Latin American immigrants with conduction abnormalities on electrocardiogram. PLOS Neglected Tropical Diseases, v. 11, n. 1, p. e0005244, 5 jan. 2017.

WEINMANN, I. Coumarins: Biology, Applications and Mode of Action. England: John Wiley & Sons, 1997.

WENIGER, B.; ROBLEDO, S.; ARANGO, G. J.; DEHARO, E.; ARAGÓN, R.; MUÑOZ, V.; CALLAPA, J.; LOBSTEIN, A.; ANTON, R. Antiprotozoal activities of Colombian plants. Journal of Ethnopharmacology, v. 78, n. 2–3, p. 193–200, dez. 2001.

WHO. WORLD HEALTH ORGANIZATION. Schistosomiasis. Disponível em: <https://www.who.int/news-room/fact-sheets/detail/schistosomiasis>. Acesso em: 22 fev. 2024.

WHO. WORLD HEALTH ORGANIZATION. World Chagas Disease Day: finding and reporting every case. 2022. Disponível em: <https://www.who.int/news/item/14-04-2022-world-chagas-disease-day-bringing-a-forgotten-disease-to-the-fore-of-global-attention>. Acesso em: 22 fev. 2024

XU, Z.; CHEN, Q.; ZHANG, Y.; LIANG, C. Coumarin-based derivatives with potential anti-HIV activity. Fitoterapia, v. 150, p. 104863, abr. 2021.

ZAID, A. N.; RAMAHI, R. AL. Depigmentation and anti-aging treatment by natural molecules. Current Pharmaceutical Design, v. 25, n. 20, p. 2292–2312, 19 set. 2023.

Downloads

Publicado

2024-04-01

Edição

2024: Science in Action

Seção

Articles