T. Catappa confers an antioxidant effect to electrospun polyvinyl alcohol nanofibers
T. Catappa confere um efeito antioxidante às nanofibras de álcool polivinílico eletrofiadas
Resumo
In this study, the healing process of full-thickness skin wounds induced in Wistar rats (n = 75) was evaluated, divided into 5 treatments: negative control, PVA nanofiber film, and PVA nanofiber film added in concentrations of 3%, 6% and 10% with lyophilized extract of T. catappa. After euthanasia of 5 animals from each of the groups, at times 7, 14 and 21 days, samples were evaluated for the presence of oxidative stress markers reduced Glutathione (GSH) and levels of lipoperoxidation (LPO), and for the histological characteristics of tissues stained with hematoxylin-eosin and azan. The results showed that, in addition to remaining on the surface of the wounds for an acceptable period, the nanofiber membrane was able to deliver the extract of T. catappa to the bed of the treated wounds and, despite not producing statistically relevant histological changes, it was observed significant antioxidant effect by reducing LPO and increasing GSH in a dose dependent manner. Thus, it is concluded that the PVA nanofiber film associated with the T. catappa extract has an antioxidant effect and can assist in wound healing
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Referências
BLAIR, Michael J. et al. Skin structure–function relationships and the wound healing response to intrinsic aging. Advances in wound care, v. 9, n. 3, p. 127-143, 2020.
BOSCARATO, Andre. G. et al. Phytochemical characterization and antimicrobial activity of terminalia catappa and its inclusion in the preparation of pva electrospun film with potential healing effect. World Journal of Pharmacy and Pharmaceutical Sciences. 2021. v.10 n.4. p.1672-1691.
BUCH, Pranali J.; CHAI, Yunrong; GOLUCH, Edgar D. Treating polymicrobial infections in chronic diabetic wounds. Clinical microbiology reviews, v. 32, n. 2, p. e00091-18, 2019.
CANCHY, Ludivine et al. Wound healing and microbiome, an unexpected relationship. Journal of the European Academy of Dermatology and Venereology, v. 37, p. 7-15, 2023.
CHEN, Xiaofeng et al. A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings. Wound Repair and Regeneration, v. 29, n. 5, p. 820-829, 2021.
CHINAKA, Chioma Nnennaya; EZEALISIJI, Kenneth Maduabuchi; AKPOFURE, Rukevwe Elohor. Phyto-chemical Characterization of the Leaf extracts of Terminalia catappa L.(Combretaceae) Using Ultra violet-Visible, Fourier transform infrared and Gas chromatography-Mass Spectroscopic techniques. Journal of Pharmacognosy and Phytochemistry, v. 7, n. 1, p. 2017-2023, 2018.
CHOI, Minji et al. Fibroblast cell derived extracellular matrix containing electrospun scaffold as a hybrid biomaterial to promote in vitro endothelial cell expansion and functionalization. Materials Science and Engineering: C, v. 120, p. 111659, 2021.
DARMAWATI, Sri et al. Antibacterial Activity of Ketapang (Terminalia cattapa L.) Leaf Extract Against Staphylococcus aureus and Pseudomonas aeruginosa Isolates of Diabetic Wounds. In: First International Conference on Medical Technology (ICoMTech 2021). Atlantis Press, 2022. p. 93-101.
DIVYA, Natarajan et al. Phytotherapeutic efficacy of the medicinal plant Terminalia catappa L. Saudi journal of biological sciences, v. 26, n. 5, p. 985-988, 2019.
DOOSTMOHAMMADI, Mohsen; FOROOTANFAR, Hamid; RAMAKRISHNA, Seeram. Regenerative medicine and drug delivery: Progress via electrospun biomaterials. Materials Science and Engineering: C, v. 109, p. 110521, 2020.
EL-RAFIE, H. M.; EL-RAFIE, M. H.; ZAHRAN, M. K. Anti-Inflammatory and Antibacterial Activities of Nanosilver-Treated Cotton Fabric Prepared From Ethanolic Extracts of Three Terminalia SpecieS. Egyptian Journal of Chemistry, v. 60, n. Conference Issue (The 8th International Conference of The Textile Research Division (ICTRD 2017), National Research Centre, Cairo 12622, Egypt.), p. 129-142, 2017.
FATHI, Heba A. et al. Electrospun vancomycin-loaded nanofibers for management of methicillin-resistant Staphylococcus aureus-induced skin infections. International Journal of Pharmaceutics, v. 586, p. 119620, 2020.
FEKRAZAD, Sepehr; SOHRABI, Mona; FEKRAZAD, Reza. Angiogenetic and anti-inflammatory effects of photobiomodulation on bone regeneration in rat: A histopathological, immunohistochemical, and molecular analysis. Journal of Photochemistry and Photobiology B: Biology, v. 238, p. 112599, 2023.
GHOSAL, Kajal et al. Novel drug delivery systems based on triaxial electrospinning based nanofibers. Reactive and Functional Polymers, v. 163, p. 104895, 2021.
GONZALEZ, Ana Cristina de Oliveira et al. Wound healing-A literature review. Anais brasileiros de dermatologia, v. 91, p. 614-620, 2016.
HADIZADEH, Mohadeseh et al. A bifunctional electrospun nanocomposite wound dressing containing surfactin and curcumin: in vitro and in vivo studies. Materials Science and Engineering: C, v. 129, p. 112362, 2021.
HARMAN, Rebecca M.; THEORET, Christine L.; VAN DE WALLE, Gerlinde R. The horse as a model for the study of cutaneous wound healing. Advances in Wound Care, v. 10, n. 7, p. 381-399, 2021.
HE, Ronghan et al. Efficacy of a synthetic biomimetic skin substitute of PLLA/gelatin nanofiber membrane in facilitating chronic cutaneous wound healing. Materials Technology, v. 35, n. 13-14, p. 872-880, 2020.
HOLLOWAY, Samantha; HARDING, Keith G. Wound dressings. Surgery (Oxford), v. 40, n. 1, p. 25-32, 2022.
HUANG, Ya-Han et al. Protective effects and mechanisms of Terminalia catappa L. methenolic extract on hydrogen-peroxide-induced oxidative stress in human skin fibroblasts. BMC complementary and alternative medicine, v. 18, n. 1, p. 1-9, 2018.
IHEAGWAM, Franklyn Nonso et al. Terminalia catappa aqueous leaf extract reverses insulin resistance, improves glucose transport and activates PI3K/AKT signalling in high fat/streptozotocin-induced diabetic rats. Scientific Reports, v. 12, n. 1, p. 10711, 2022.
JIANG, Zhen‐Yue; WOOLLARD, Alison CS; WOLFF, Simon P. Lipid hydroperoxide measurement by oxidation of Fe2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method. Lipids, v. 26, n. 10, p. 853-856, 1991.
JORGENSEN, Adam M. et al. Advances in Skin Tissue Engineering and Regenerative Medicine. Journal of Burn Care & Research, v. 44, n. Supplement_1, p. S33-S41, 2023.
KILIÇ, Ismail; YEŞILOĞLU, Yeşim. Spectroscopic studies on the antioxidant activity of p-coumaric acid. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 115, p. 719-724, 2013.
KOPROSKI, Letícia; PACHALY, José R. Contenção farmacológica do rato-do-bambu (Dactylomys dactylinus Desmarest, 1817) e relato de ocorrência da espécie na região Sul do Brasil. Pesquisa Veterinária Brasileira, v. 37, p. 608-612, 2017.
KOSYKH, Anastasiya V.; TERESHINA, Maria B.; GURSKAYA, Nadya G. Potential Role of AGR2 for Mammalian Skin Wound Healing. International Journal of Molecular Sciences, v. 24, n. 9, p. 7895, 2023.
LEVENSON, S. M. et al. Some studies of wound healing: experimental methods, effect of ascorbic acid and effect of deuterium oxide. Journal of Trauma and Acute Care Surgery, v. 4, n. 5, p. 543-566, 1964.
LUO, Hua et al. Paracrine effects of adipose-derived stem cells in cutaneous wound healing in streptozotocin-induced diabetic rats. Journal of Wound Care, v. 31, n. Sup3, p. S29-S38, 2022.
MAHMUD, Md Musavvir et al. Controlled release of curcumin from electrospun fiber mats with antibacterial activity. Journal of Drug Delivery Science and Technology, v. 55, p. 101386, 2020.
MININEL, Francisco José et al. Characterization and quantification of compounds in the hydroalcoholic extract of the leaves from Terminalia catappa Linn.(Combretaceae) and their mutagenic activity. Evidence-Based Complementary and Alternative Medicine, v. 2014, 2014.
MOGASHOA, M. M.; MASOKO, P.; ELOFF, J. N. Different Combretum molle (Combretaceae) leaf extracts contain several different antifungal and antibacterial compounds. South African Journal of Botany, v. 126, p. 322-327, 2019.
MOHAMMED, Hamdoon A. et al. Bio-evaluation of the wound healing activity of Artemisia judaica L. as part of the plant’s use in traditional medicine; Phytochemical, antioxidant, anti-inflammatory, and antibiofilm properties of the plant’s essential oils. Antioxidants, v. 11, n. 2, p. 332, 2022.
MONIKA, Prakash et al. Myofibroblast progeny in wound biology and wound healing studies. Wound Repair and Regeneration, v. 29, n. 4, p. 531-547, 2021.
MOURO, Cláudia; FANGUEIRO, Raul; GOUVEIA, Isabel C. Preparation and characterization of electrospun double-layered nanocomposites membranes as a carrier for Centella asiatica (L.). Polymers, v. 12, n. 11, p. 2653, 2020.
NUGROHO, Rudy Agung et al. Wound healing potency of Terminalia catappa in mice (Mus musculus). EurAsian Journal of BioSciences, v. 13, n. 2, p. 2337-2342, 2019.
RAJHI, Imene et al. Assessment of dehulling effect on volatiles, phenolic compounds and antioxidant activities of faba bean seeds and flours. South African Journal of Botany, v. 147, p. 741-753, 2022.
RAVI, Lokesh. A review on medicinal potential of Terminalia catappa. International Journal of Green Pharmacy (IJGP), v. 14, n. 03, 2020.
SCHNEIDER, Rodrigo et al. Biocompatible electrospun nanofibers containing cloxacillin: Antibacterial activity and effect of pH on the release profile. Reactive and Functional Polymers, v. 132, p. 26-35, 2018.
SEDLAK, Jozef; LINDSAY, Raymond H. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical biochemistry, v. 25, p. 192-205, 1968.
SHAHID, Md Abdus et al. Antibacterial wound dressing electrospun nanofibrous material from polyvinyl alcohol, honey and Curcumin longa extract. Journal of Industrial Textiles, v. 51, n. 3, p. 455-469, 2021.
STREETER, Benjamin W. et al. Electrospun nanofiber-based patches for the delivery of cardiac progenitor cells. ACS applied materials & interfaces, v. 11, n. 20, p. 18242-18253, 2019.
TABAKOGLU, Hasim O. et al. Assessment of circular wound healing in rats after exposure to 808‐nm laser pulses during specific healing phases. Lasers in surgery and medicine, v. 48, n. 4, p. 409-415, 2016.
TERÇAS, Analucia G. et al. Phytochemical characterization of Terminalia catappa linn. Extracts and their antifungal activities against candida spp. Frontiers in Microbiology, v. 8, p. 595, 2017.
VENKATALAKSHMI, P.; BRINDHA, P. In vivo Immunomodulatory Potential of Aqueous Extract of Different Parts of Terminalia catappa L. Indian Journal of Pharmaceutical Sciences, v. 83, n. 3, p. 584-593, 2021.
WILKINSON, Holly N.; HARDMAN, Matthew J. Wound healing: Cellular mechanisms and pathological outcomes. Open biology, v. 10, n. 9, p. 200223, 2020.
XIE, Xianrui et al. Electrospinning nanofiber scaffolds for soft and hard tissue regeneration. Journal of Materials Science & Technology, v. 59, p. 243-261, 2020.
XUE, Jiajia et al. Electrospun nanofibers: new concepts, materials, and applications. Accounts of chemical research, v. 50, n. 8, p. 1976-1987, 2017.
YADAV, Sarita et al. Terminalia catappa Linn.: A TREASURY OF PHARMACOLOGICAL BENEFITS. UTTAR PRADESH JOURNAL OF ZOOLOGY, p. 1386-1396, 2021.
YAZDANBAKHSH, M. F. et al. The effect of impregnated alpha-cellulose nanofibers with ciprofloxacin hydrochloride on staphylococcus aureus in vitro and healing process of wound in rat. Regenerative Engineering and Translational Medicine, v. 4, p. 247-256, 2018.
ZHANG, Xi et al. Nanoengineered electrospun fibers and their biomedical applications: a review. Nanocomposites, v. 7, n. 1, p. 1-34, 2021.
