Cellular profile of the skin and ocular surface in healthy shih-tzu dogs
Perfil citológico da pele e superfície ocular de cães da raça shih-tzu saudáveis
Palavras-chave:
Canids, Cytopathology, Dermatology, Morphology, Ocular healthResumo
Cytology involves analyzing individual cells and is crucial for diagnosing skin and ocular surface diseases. The skin acts as a barrier against various threats and communicates with the immune system via receptors. The conjunctiva also provides protection to the eyes. This study aimed to describe the cytological profiles of healthy Shih-Tzu dogs' skin and ocular surfaces, assessing how age impacts these profiles. Materials for cytological analysis were collected using a nylon microbrush and evaluated with the Schirmer test. Results showed lymphocytes were predominant in ocular cytology (RE - 47%, LE - 41.93%), while polymorphonuclear cells dominated in nasal fold (62%) and palmar pad cytologies (100%). Superficial cells were prevalent in all regions (RE 83.11%, LE 90.52%, palmar pads 100%, nasal fold 96.91%). Malassezia spp was detected in all areas. This research underscores the cellular variations across different anatomical sites and provides insights into normal cytological patterns in these dogs.
Downloads
Referências
AFSHAR, M.; GALLO, R. L.; Innate immune defense system of the skin. Advances in Veterinary Dermatology – The Authors. Journal compilation, ESVD and ACVD, 2013.
AZEVEDO G. M., SOUZA, A. P. PORTELA R. A., DANTAS, E.S., SILVA R. M. N., NETO J. E. AVALIAÇÃO CITOLÓGICA DA CONJUNTIVA DE CÃES CLINICAMENTE SADIO PELO MÉTODO PANÓTICO. MedVep, Revista Científica de Medicina Veterinária Pequenos Animais e Animais de estimação 7(3); 473-477, 2009.
BOLZAN, A. A., BRUNELLI, A. T. J., CASTRO, M. B., SOUZA, M. A., SOUZA, J. L., LAUS, J. L. Conjunctival impression cytology in dogs. Veterinary Ophthalmology, Oxford, v. 8, n. 6, p. 401-405, 2005.
BORGES, R.F.; CARDOSO, K.C.F.; BOLZAN, A.A.; MOMO, C.; HONSHO, C.S. Estudo comparativo de métodos de coleta e coloração para citologia conjuntival em cães normais. Vet e Zootec.19, (3):381-391, 2012.
BRON AJ, DE PAIVA CS, CHAUHAN SK, BONINI S, GABISON EE, JAIN S, KNOP E, MARKOULLI M, OGAWA Y, PEREZ V, UCHINO Y, YOKOI N, ZOUKHRI D, SULLIVAN DA. TFOS DEWS II pathophysiology report. Ocul Surf. 2017 Jul;15(3):438-510. doi: 10.1016/j.jtos.2017.05.011. Epub 2017 Jul 20. Erratum in: Ocul Surf. 2019 Oct;17(4):842. PMID: 28736340.
CHIURCO, E. C. Isolation and production of enzymes by Malassezia pachydermatis from dogs with otitis or dermatitis and healthy dogs. Master's Thesis. - 2016. 54 f.: color il.
COWELL, R. L. Diagnóstico citológico e hematologia de cães e gatos. [translation Adriana de Siqueira Rosinelli...; scientific review Alexander Welker Biondo, Rafael Felipe da Costa Vieira]. MedVet, 3.ed. p36, 2009.
DIAZ, L., CASTELLA, G., BRAGULAT, M. R. GALLART, A. P., SANSEVERINO, W., CABANES, F.J., Study of the variation of Malassezia load in the interdigital fold of dogs with pododermatitis. Comunicações de Pesquisa Veterinária (2023) 47:385–396 https:// doi.org/10.1007/s11259-022-09951-2.
GASPARETTO, N. D.; TREVISAN, Y. P. A.; ALMEIDA, N. B.; NEVES, R. C. S. M.; ALMEIDA, A. B. P. F.; DUTRA, V.; COLODEL, E. M. SOUSA, V. R. F., Prevalence of non-neoplastic skin diseases in dogs in the municipality of Cuiabá, Mato Grosso - Pesquisa Veterinária Brasileira, 33, 2013 33(3)), p359–362.
HASHITOMO, D. Tissue-resident macrophges self-maintain locally throughout adult life with minimal contribution from circulating monocytes. Immunity, v.38, n4 p.792-804,2013.
HOBI, S.; CAFARCHIA, C.; ROMANO, V.; BARRS, VR Malassezia: Zoonotic Implications, Parallels and Differences in Colonization and Disease in Humans and Animals. Fungi 2022, 8(7), 708; https://doi.org/10.3390/jof8070708.
JUNQUEIRA, L. C.; CARNEIRO, J. Histologia Básica: Texto e Atlas. 14th ed. Rio de Janeiro: Grupo GEN, 2023. p. 99.
KOVALCUKA L, SARPIO L, NIKOLAJENKO M. Comparison of five conjunctival cytology sampling methods in normal cat eyes. Vet World. 2023 Apr;16(4):779-785. doi: 10.14202/vetworld.2023.779-785.
LIMA, F. B. ORIÁ A. P. MENEZES I. D. S. RAPOSO, A. C. S. JUNIOR, D. C. G. Exfoliative cytology in dogs with keratoconjunctivitis sicca, Enciclopédia Biosfera, Centro Científico Conhecer - Goiânia, v.10, n.18; 2014.
MARTIN, C. L. Anamnesis and the Ophthalmic Examination. London: Manson Publishing Ltd, p. 15, 2010.
MCGAVIN, M.D; ZACHARY J.F Bases da Patologia em Veterinária, 6ed, Rio de Janeiro: Elsevier, 2018, p134.
MORAIS, T.L. Ophthalmological evaluation of healthy Gir and Nelore cattle. Master's Thesis (Dissertation). Universidade Federal de Goiás. Escola de Veterinária e Zootecnia. Programa de pós-graduação em ciência animal. 2016.
PERAZZI A, BONSEMBIANTE F, GELAIN ME, PATRUNO M, DI IORIO E, MIGLIORATI A, IACOPETTI I. Cytology of healthy canine and feline ocular surface: comparison between cytobrush and impression technique. Vet Clin Pathol "in press" 2017.
PUTZ, Carla. Ophthalmology - Basic Sciences. Grupo GEN, 2017. E-book. ISBN 9788595152199. Available at: https://integrada.minhabiblioteca.com.br/#/books/9788595152199/. Accessed on: Feb 12, 2024.
RASKIN, R. Atlas of cytology of dogs and cats. São Paulo: Roca, p. 365-369, 2003.
ROSA, L. D.; GOMES, F. T.; BARROS, T. A.; CARVALHO, A. V. Presence of Malassezia sp. in dogs from the Zoonosis Control Unit of Guaraí (TO) and its relationship with humans. Health of Humans, v.1, n.1, p.1-8, 2019. DOI: http://doi.org/10.6008/CBPCxxxx-xxxx.2019.001.00xx
SANTOS, L. G. F., ALMEIDA, A. B. P. F., SILVA, M. C., OLIVEIRA, J. T., DUTRA, V., SOUSA, V., R. F. Conjunctival microbiota of healthy dogs and dogs with ophthalmic disorders. Acta Scientiae Veterinariae. 37(2): 165-169, 2009.
SANTOS, A. J., VIEIRA, M. C. G., LIMA P. P. A. OLIVEIRA L. R. C.; CARDINOT, C. B.; ROCHA, T. V. P.; LANNA, L. L. FRANCISCATO, C. Prevalence of microorganisms and mites found in dermatological and otological samples of dogs and cats. Revista Brasileira de Higiene e Sanidade Animal, Fortaleza, v. 14, n. 3, p. 1-11, Jul./Sep. 2020.
VENÂNCIO, S. A. S. VIEIRA, A. B., ALENCAR, N. X., SOARES, A. M. B. Evaluation of the cytological brushing technique for collecting conjunctival cells in healthy cats: comparison between the palpebral face of the nictitating membrane and palpebral conjunctiva. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 32, n. 11, p. 1199-1204, 2012.