Considerations on the authenticity and nutritional aspects of green coffee products consumed in Brazil
Considerações sobre os aspectos de autenticidade e nutricionais de produtos de café verde consumidos no Brasil
Palavras-chave:
Chlorogenic acid, Green coffee capsules, Ochratoxin A, Food supplementResumo
Several benefits have been attributed to the consumption of green coffee, in the form of capsules or teas, mainly due to its high content of phenolic acids, which allegedly show antioxidant, antimutagenic, and anticarcinogenic activities in vitro. Chlorogenic acid is the phenolic compound present in greater proportion in coffee and the consumption of green coffee extract has been associated with health and loss of weight. However, beyond these bioactive compounds with a healthy appeal, other compounds which can have deleterious health effects should be considered in the consumption of these extracts, such as the presence of contaminants (ochratoxin A and its producing fungi), phytates, and tannins, which exhibit anti-nutritional characteristics. In that sense, this manuscript seeks to assess the composition of commercial green coffee extracts, analyzing their authenticity, nutritional and anti-nutritional aspects, to provide technical information for a qualified discussion about the effective healthiness of the consumption of commercial green coffee extracts. Nine samples of green coffee food supplements were selected and a product was prepared to serve as a standard. It was possible to identify coffee DNA in six of the commercial samples of food supplements. The products had antioxidant activity between 33 and 2408 µmol of Trolox/g. It was possible to identify chlorogenic acid in all samples with concentrations ranging from 0.023 - 20 mg / g of coffee. Six samples presented positive values for ochratoxin A, despite being within the limits of the coffee roasted legislation, there is a certain concern about the safety of these supplements.
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ABNT NBR / ISO 17025: 2005
Amigoni, L., et al. “Green Coffee Extract Enhances Oxidative Stress Resistance and Delays Aging in Caenorhabditis Elegans.” Journal of Functional Foods, vol. 33, 1 June 2017, pp. 297–306, https://doi.org/10.1016/j.jff.2017.03.056.
ANVISA, Brazil. Instrução normativa - IN Nº 160, de 1° de julho de 2022 (Publicada no DOU nº 126, de 6 de julho de 2022) Estabelece os limites máximos tolerados (LMT) de contaminantes em alimentos. 2022. https://antigo.anvisa.gov.br/documents/10181/2718376/IN_160_2022_.pdf
ANVISA, Brazil. Resolution RDC Nº 360, 23 DE DEZEMBRO DE 2003. https://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2003/res0360_23_12_2003.html
AOAC (Association of Official Analytical Chemists). Official Methods of Analysis of AOAC International. 17th ed. Gaithersburg, 2014.
AOAC. Association of official analytical chemists, official methods of analysis. 18. Washington, DC: AOAC International; 2005.
AOAC. Association of official analytical chemists, official methods of analysis - Method 986.11. Phytate in Foods, 18 ed., 3ª rev, 2010.
Arlorio, M. et al. “Detection of Hazelnut (Corylus Spp.) in Processed Foods Using Real-Time PCR.” Food Control, vol. 18, no. 2, Feb. 2007, pp. 140–148, https://doi.org/10.1016/j.foodcont.2005.09.005.
Batista, L.R. et al. “Toxigenic Fungi Associated with Processed (Green) Coffee Beans (Coffea Arabica L.).” International Journal of Food Microbiology, vol. 85, no. 3, 25 Aug. 2003, pp. 293–300, https://doi.org/10.1016/s0168-1605(02)00539-1.
Bauer, D. et al. “Effect of Roasting Levels and Drying Process of Coffea Canephora on the Quality of Bioactive Compounds and Cytotoxicity.” International Journal of Molecular Sciences, vol. 19, no. 11, 31 Oct. 2018, p. 3407, https://doi.org/10.3390/ijms19113407.
Belguidoum, K. et al. “HPLC Coupled to UV–Vis Detection for Quantitative Determination of Phenolic Compounds and Caffeine in Different Brands of Coffee in the Algerian Market.” Journal of the Taiwan Institute of Chemical Engineers, vol. 45, no. 4, July 2014, pp. 1314–1320, https://doi.org/10.1016/j.jtice.2014.03.014.
Broadhurst, R.B. and Jones, W.T. “Analysis of Condensed Tannins Using Acidified Vanillin.” Journal of the Science of Food and Agriculture, vol. 29, no. 9, Sept. 1978, pp. 788–794, https://doi.org/10.1002/jsfa.2740290908.
CAC (Codex Alimentarius Commission). Joint FAO/WHO Food Standards Programme, Codex Committee on Contaminants in Foods Thirty-fifth Session, Arusha, Tanzania, 17–21 March 2003, Document Reference CX/FAC 03/18.
Copetti, M.V. et al. “Comparison of Different Culture Media for Mycological Evaluation of Commercial Pet Food.” Acta Scientiae Veterinariae, vol. 37, no. 4, 30 Mar. 2018, p. 329, https://doi.org/10.22456/1679-9216.16392.
Costa, A.S.G. et al. “Teas, Dietary Supplements and Fruit Juices: A Comparative Study Regarding Antioxidant Activity and Bioactive Compounds.” LWT - Food Science and Technology, vol. 49, no. 2, Dec. 2012, pp. 324–328, https://doi.org/10.1016/j.lwt.2012.02.030.
Costa, J. et al. “High Resolution Melting Analysis as a New Approach to Detect Almond DNA Encoding for Pru Du 5 Allergen in Foods.” Food Chemistry, vol. 133, no. 3, Aug. 2012, pp. 1062–1069, https://doi.org/10.1016/j.foodchem.2012.01.077.
Couto, C.C. et al. “Coffea Arabica and C. Canephora Discrimination in Roasted and Ground Coffee from Reference Material Candidates by Real-Time PCR.” Food Research International, vol. 115, Jan. 2019, pp. 227–233, https://doi.org/10.1016/j.foodres.2018.08.086.
Crozier, A. et al. “Dietary Phenolics: Chemistry, Bioavailability and Effects on Health.” Natural Product Reports, vol. 26, no. 8, 2009, p. 1001, https://doi.org/10.1039/b802662a.
Dellalibera, O., et al. “Le Svetol ®, Un Extrait de Café Vert Décaféiné, Induit Une Perte de Poids et Augmente Le Ratio Masse Maigre Sur Masse Grasse Chez Des Volontaires En Surcharge Pondérale.” Phytothérapie, vol. 4, no. 4, Nov. 2006, pp. 194–197, https://doi.org/10.1007/s10298-006-0181-7.
Dziki, D. et al. “Ground Green Coffee Beans as a Functional Food Supplement – Preliminary Study.” LWT - Food Science and Technology, vol. 63, no. 1, Sept. 2015, pp. 691–699, https://doi.org/10.1016/j.lwt.2015.03.076.
Ferreira, T. et al. “Using Real-Time PCR as a Tool for Monitoring the Authenticity of Commercial Coffees.” Food Chemistry, vol. 199, May 2016, pp. 433–438, https://doi.org/10.1016/j.foodchem.2015.12.045.
Gaascht, François, et al. “Coffee Provides a Natural Multitarget Pharmacopeia against the Hallmarks of Cancer.” Genes & Nutrition, vol. 10, no. 6, Nov. 2015, https://doi.org/10.1007/s12263-015-0501-3.
Hečimović, Ivana, et al. “Comparative Study of Polyphenols and Caffeine in Different Coffee Varieties Affected by the Degree of Roasting.” Food Chemistry, vol. 129, no. 3, Dec. 2011, pp. 991–1000, https://doi.org/10.1016/j.foodchem.2011.05.059.
Hendre, P.S. and Aggarwal, R.K. “Development of Genic and Genomic SSR Markers of Robusta Coffee (Coffea Canephora Pierre Ex A. Froehner).” PLoS ONE, vol. 9, no. 12, 2 Dec. 2014, p. e113661, https://doi.org/10.1371/journal.pone.0113661.
ICO. International coffee organization. https: //www.ico.org/trade_statistics.asp?section=Statistics, 2023 (accessed 13 June 2023).
Jeszka-Skowron, Magdalena, et al. “Relationship between Antioxidant Capacity, Chlorogenic Acids and Elemental Composition of Green Coffee.” LWT, vol. 73, Nov. 2016, pp. 243–250, https://doi.org/10.1016/j.lwt.2016.06.018.
Lee, Jae-Hwang, et al. “Specific PCR Assays to Determine Bovine, Porcine, Fish and Plant Origin of Gelatin Capsules of Dietary Supplements.” Food Chemistry, vol. 211, 15 Nov. 2016, pp. 253–259, https://doi.org/10.1016/j.foodchem.2016.05.060.
Liang, Ningjian, et al. “Interactions between Major Chlorogenic Acid Isomers and Chemical Changes in Coffee Brew That Affect Antioxidant Activities.” Food Chemistry, vol. 213, Dec. 2016, pp. 251–259, https://doi.org/10.1016/j.foodchem.2016.06.041.
Lima, A. R., Pereira, R. G. F. A., Abrahão, S. A., Duarte, S. M. da S., & Paula, F. B. de A. (2010). Compostos bioativos do café: atividade antioxidante in vitro do café verde e torrado antes e após a descafeinação. Química Nova, 33(1), 20–24. https://doi.org/10.1590/s0100-40422010000100004
Lima, M.B., et al. “Turbidimetric and Photometric Determination of Total Tannins in Tea Using a Micro-Flow-Batch Analyzer.” Talanta, vol. 88, 15 Jan. 2012, pp. 717–723, https://doi.org/10.1016/j.talanta.2011.11.076.
Liu, Q, et al. “Optimization of Ultrasonic-Assisted Extraction of Chlorogenic Acid from Folium Eucommiae and Evaluation of Its Antioxidant Activity.” Journal of Medicinal Plants Research, vol. 4, no. 23, 2010, pp. 2503–2511, academicjournals.org/article/article1380705215_Liu%20et%20al.pdf. Accessed 7 Oct. 2023.
Mahabir, S. “Methodological Challenges Conducting Epidemiological Research on Nutraceuticals in Health and Disease.” PharmaNutrition, vol. 2, no. 3, July 2014, pp. 120–125, https://doi.org/10.1016/j.phanu.2013.06.002.
Manzano, M. et al. “A Molecular Method to Detect Bacillus Cereus from a Coffee Concentrate Sample Used in Industrial Preparations.” Journal of Applied Microbiology, vol. 95, no. 6, 1 Dec. 2003, pp. 1361–1366, https://doi.org/10.1046/j.1365-2672.2003.02120.x.
Martellossi, C. et al. “DNA Extraction and Analysis from Processed Coffee Beans.” Journal of Agricultural and Food Chemistry, vol. 53, no. 22, 4 Oct. 2005, pp. 8432–8436, https://doi.org/10.1021/jf050776p.
Morgano, Marcelo Antonio, et al. “Determinação de Minerais Em Café Cru.” Ciência E Tecnologia de Alimentos, vol. 22, no. 1, Jan. 2002, pp. 19–23, https://doi.org/10.1590/s0101-20612002000100004.
Murray, M.G., and Thompson, W.F. “Rapid Isolation of High Molecular Weight Plant DNA.” Nucleic Acids Research, vol. 8, no. 19, 1980, pp. 4321–4326, https://doi.org/10.1093/nar/8.19.4321.
Narita, Yusaku, and Kuniyo Inouye. “High Antioxidant Activity of Coffee Silverskin Extracts Obtained by the Treatment of Coffee Silverskin with Subcritical Water.” Food Chemistry, vol. 135, no. 3, Dec. 2012, pp. 943–949, https://doi.org/10.1016/j.foodchem.2012.05.078.
Nóbile, P.M. et al. “Transcriptional Profile of Genes Involved in the Biosynthesis of Phytate and Ferritin in Coffea.” Journal of Agricultural and Food Chemistry, vol. 58, no. 6, 22 Feb. 2010, pp. 3479–3487, https://doi.org/10.1021/jf9043088.
Paulino De Moraes, M. H., Luchese, R.H. “Ochratoxin a on Green Coffee: Influence of Harvest and Drying Processing Procedures.” Journal of Agricultural and Food Chemistry, vol. 51, no. 19, 6 Aug. 2003, pp. 5824–5828, https://doi.org/10.1021/jf026248k.
Pawar, R. et al. “Assessment of the Authenticity of Herbal Dietary Supplements: Comparison of Chemical and DNA Barcoding Methods.” Planta Medica, vol. 83, no. 11, 28 Apr. 2017, pp. 921–936, https://doi.org/10.1055/s-0043-107881.
Pitt, John I., and Ailsa D. Hocking. Fungi and Food Spoilage. Boston, MA, Springer US, 2009.
Popova, A. and Mihaylova, D. “Antinutrients in Plant-Based Foods: A Review.” The Open Biotechnology Journal, vol. 13, no. 1, 29 July 2019, pp. 68–76, https://doi.org/10.2174/1874070701913010068.
Powell, K. A. et al. The Genus Aspergillus. Springer Science & Business Media, 29 June 2013.
Ramirez-Coronel, M. A. et al. “Characterization and Estimation of Proanthocyanidins and Other Phenolics in Coffee Pulp (Coffea Arabica) by Thiolysis−High-Performance Liquid Chromatography.” Journal of Agricultural and Food Chemistry, vol. 52, no. 5, 11 Feb. 2004, pp. 1344–1349, https://doi.org/10.1021/jf035208t.
Rosa, J.S., Freitas-Silva, O., Campos, R.S., Castro, I.M., Teixeira, A.S. Determination of pesticides and Ochratoxin A in arabica coffee under different roasting levels. (Determinação de agrotóxicos e ocratoxina A em café arábica sob diferentes Níveis de Torrefação). Rio de Janeiro. Embrapa Agroindústria de Alimentos, 2019.18 p. – (Boletim de Pesquisa e Desenvolvimento / Embrapa Agroindústria de Alimentos, ISSN 0101-630X; 29). https://www.infoteca.cnptia.embrapa.br/bitstream/doc/1112112/1/BPD292019ocratoxina.pdf
Samson, R.A., et al. “Phylogeny, Identification and Nomenclature of the Genus Aspergillus.” Studies in Mycology, vol. 78, June 2014, pp. 141–173, https://doi.org/10.1016/j.simyco.2014.07.004.
Santato, Alessandro, et al. “Using Elemental Profiles and Stable Isotopes to Trace the Origin of Green Coffee Beans on the Global Market.” Journal of Mass Spectrometry, vol. 47, no. 9, Sept. 2012, pp. 1132–1140, https://doi.org/10.1002/jms.3018.
Schrenk, D. et al. “Risk Assessment of Ochratoxin a in Food.” EFSA Journal, vol. 18, no. 5, May 2020, https://doi.org/10.2903/j.efsa.2020.6113.
Şemen, Sevcan, et al. “Elemental Composition of Green Coffee and Its Contribution to Dietary Intake.” Food Chemistry, vol. 215, Jan. 2017, pp. 92–100, https://doi.org/10.1016/j.foodchem.2016.07.176.
Shimoda, Hiroshi, et al. “Inhibitory Effect of Green Coffee Bean Extract on Fat Accumulation and Body Weight Gain in Mice.” BMC Complementary and Alternative Medicine, vol. 6, no. 1, 17 Mar. 2006, https://doi.org/10.1186/1472-6882-6-9.
Silva, M.R. and Silva, M.A.A.P. “Aspectos Nutricionais de Fitatos E Taninos.” Revista de Nutrição, vol. 12, no. 1, Apr. 1999, pp. 21–32, https://doi.org/10.1590/s1415-52731999000100002.
Souza, Laurent dos Santos de, et al. “Effect of the Roasting Levels of Coffea Arabica L. Extracts on Their Potential Antioxidant Capacity and Antiproliferative Activity in Human Prostate Cancer Cells.” RSC Advances, vol. 10, no. 50, 2020, pp. 30115–30126, https://doi.org/10.1039/d0ra01179g. Accessed 26 Nov. 2022.
Ukers, W.H. The Chemistry of the Coffee Bean. In: UKERS, W.H. (Ed.) All About Coffee. 2ed. New York: Inter-American Copyright Union, 1976. Cap. 24, p. 293.
Vaclavik, Lukas, et al. “Determination of Multiple Mycotoxins in Dietary Supplements Containing Green Coffee Bean Extracts Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS).” Journal of Agricultural and Food Chemistry, vol. 61, no. 20, 22 May 2013, pp. 4822–4830, pubmed.ncbi.nlm.nih.gov/23631685/, https://doi.org/10.1021/jf401139u.
Zulueta, A., et al. “ORAC and TEAC Assays Comparison to Measure the Antioxidant Capacity of Food Products.” Food Chemistry, vol. 114, no. 1, May 2009, pp. 310–316, https://doi.org/10.1016/j.foodchem.2008.09.033.